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M94A0634.TXT
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1994-10-21
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Document 0634
DOCN M94A0634
TI A cis-acting repressive sequence that overlaps the Rev-responsive
element of HIV-1 regulates nuclear retention of env mRNAs independently
of splice signals.
DT 9412
AU Brighty DW; Rosenberg M; SmithKline Beecham Pharmaceuticals, King of
Prussia, Pennsylvania; 19406-0939.
SO Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:67 (abstract no. FB1).
Unique Identifier : AIDSLINE ASHM5/94349021
AB The Rev protein of HIV-1 binds to an RNA structure, the Rev-Responsive
Element (RRE), and enhances expression of the viral structural genes by
relieving the nuclear sequestration of incompletely spliced viral
transcripts. It has been due to the activity of cis-acting repressive
sequence (CRS) elements and to inefficient splicing signals. We have
demonstrated that expression of the HIV-1 envelope gene in transfected
Drosophila cells is also completely dependent upon co-expression of Rev
and hence, the mechanism of nuclear retention and Rev regulation is
highly conserved in higher cell systems. We use the Drosophila system to
identify a major CRS element which overlaps the RRE and is responsible
for the nuclear entrapment and Rev-dependent expression of HIV-1
envelope encoding mRNAs. Moreover, the splice signals spanning env are
not required for nuclear retention or Rev-dependent trans-activation of
env mRNAs. Thus the RRE and its associated complex RNA structure appear
necessary and sufficient for both the repressive and known
trans-activation effects of Rev regulation.
DE Animal Cell Nucleus/PHYSIOLOGY Cells, Cultured Drosophila/GENETICS
Gene Products, env/*GENETICS Gene Products, rev/*GENETICS Genetic
Complementation Test Human HIV-1/*GENETICS Repressor
Proteins/*GENETICS RNA Splicing/*GENETICS Signal
Transduction/*GENETICS Transfection/GENETICS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).